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Important progress in product development – simultaneous staining in the same section, with high degree of detail

Lumito is developing a novel method for high-contrast imaging without background signal. The first application is developed for tissue diagnostics and digital pathology. In order to visualize cells containing Her2, which is a common marker in breast cancer diagnostics, Lumito is now presenting new images of breast tissues stained with the Company’s Her2-UCNP reagent.

Lumito’s technology makes it possible to stain tissue with nanoparticle-based reagents, and thus to create images with the company’s proprietary analysis instrument. Two of the major advantages are the ability to create imaging with high contrast and detail, and the ability to use both Lumito’s reagent and traditional reagents to stain the same tissue section.

The images show a degree of detail comparable to the one achieved with traditional staining methods. Elimination of all insignificant information from background signal and autofluorescence provides significantly better conditions for quantitative automated analysis based on, for example, AI algorithms. Lumito’s technology has the advantage that there are only signals from the wanted markers – in this case breast cancer marker Her2.

When you examine the Her2 imaging based on Lumito’s technology, you can readily understand the opportunities it opens for pathologists when they are able to see only the tissue they are interested in. The technology further opens the door for image processing, which may help facilitate automated diagnostics in the future.

Lumito is also publishing cell imagery where UCNP staining and traditional H&E staining have been combined in the same section. The user may then choose to perform analysis on the images separately, or on the aggregate image.

Lumito gratefully acknowledges the contribution of Dr. Hans-Heiner Gorris, University of Regensburg, whose laboratory provided the UCNP reagent above to Lumito.

Figure 1. Lumito’s UCNP staining of HER2 marker in breast tissue. The image is an example of how Lumito’s technology only reproduce the wanted markers and leave out tissue fluorescence. Searched areas are easily located in each sample due to the contrast difference between marker (white) and background (black)

Figure 2. Image of UCNP-stained HER2 marker in breast tissue. The color is easily edited digitally and enables the emphasis of areas of interest in each sample.

Figure 3. Combined UCNP and H&E staining in the same cell section.
Lumito’s technology supports the performance of both UCNP and H&E staining in the same tissue sample. The user may then choose to perform analysis on the images separately, or on the aggregate image. Image A shows a UCNP-based Her2 stain of BT 474, a breast cancer cell line. In image B, the same section is shown with a standard H&E stain. Image C shows the aggregate image. The user may choose to perform analysis on both images separately, or, to improve accuracy and diagnostic reliability, on the aggregate image as shown here. (The identified cancer cells are visible in yellow).

Lumito gratefully acknowledges the contribution of Dr. Hans-Heiner Gorris, University of Regensburg, whose laboratory provided the UCNP reagent above to Lumito.