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Innovative SCIZYS by Lumito

SCIZYS by Lumito is the name of Lumito’s product solution consisting of a  scanner and UCNP reagents (Upconverting Nano Particle staining liquids). The ambition of Lumito is to launch this unique system, for immunohistochemical (IHC)l imaging of protein markers in tissue sections in the first part of 2023 towards the research laboratories segment, followed by a clinical product.

SCIZYS by Lumito offers researchers and pathologists with an enhanced multiplexing capability by contributing with an “invisible” immunohistochemical channel that does not affect the visualization of tissue morphology or other chromogen based IHC stainings in the tissue. The more markers that are imaged, the more specific information is provided for the specific case.

SCIZYS by Lumito allows simultaneous tissue analysis in two modalities, bright field imaging for the overall histomorphology and for the localization of protein markers with IHC. 

The images are stored in two separate channels but are spatially fully coordinated and can be overlaid. The technique allows unlimited analysis of histological parameters in a tissue section. 

The unique feature of SCIZYS is that the IHC reaction is “invisible but can be visualized by pressing a key. This means that the specific IHC information at the cellular level can be obtained  simultaneously while keeping the histological information intact  during the process. This means that no consecutive tissue section would be required for a separate IHC reaction, which can save precious tissue, time, and laboratory resources.

 

Tissue images showing protein expression in pig skin

The images above, A-C, show that Lumito’s technique can be used to detect protein expression in specific tissue structures, such as skin. Image A showing immunostaining for the protein CDH1 on porcine skin, showing membranous immunostaining of keratinocytes within the epidermis. Imaging overlay has been made with the nuclear counterstain hematoxylin.

 


Fig 1 and Fig 2

 

Immunostaining of synaptic structures in pig cerebral cortex

Figure 1 above shows immunostaining of synaptic structures in cerebral cortex. This has been done by Lumito’s UCNP-based immunostaining of the protein SYP1 (green). The blue counterstain is general nuclear staining with hematoxylin.

Figure 2 shows a detail from Figure 1. The image has been created to show a high-resolution image of marker protein in tissue produced using Lumito’s technique. The SYP1 protein is used as a diagnostic biomarker for tissue damage in the brain.

 

 

Innovative digital imaging technology meets increasing number of biopsies

Lumito presents an innovative novel imaging technology. Using the company’s digital images, pathologists and researchers can obtain a more complete histological basis for analyses and clinical diagnostics.

We want to offer a powerful tool to meet the demands of rapid and safe tissue diagnostics. The plan is to launch our first product for research labs in the first part of 2032, followed by a clinical product.

The scanner also takes pictures of traditional hematoxylin and eosin-stained tissue sections, as well as of chromogen-based immunohistochemistry (IHC).

Higher analysis quality and shorter analysis times

The technology makes it possible to obtain images with higher contrast, increased dynamic range and reduced non-specific background. By achieving higher analysis quality and shorter analysis times, there is potential to significantly improve the diagnostics of tissue samples.

It is also possible to switch between visualization of the morphology and our unique UCNP IHC marking. The flexibility of the system also allows visualization of both readings simultaneously, superimposed on the same image. Altogether, this provides more detailed information, without the problems of overlapping and non-specific marking obtained with traditional methodology.

Lumito has recently completed a pre-study together with Umeå University, led by Associate Professor Daniel Öhlund, with good results. The research team has identified how the company’s UCNP technology can be used to improve the ability to visualize protein expression in pancreatic cancer.

 

“Using Lumito’s imaging technology, we have investigated, among other things, whether a certain protein spreads via secretion from the cancer cells to the tumor’s supporting tissue, the tumor stroma. Lumito’s technology has led to greater possibilities, compared to other immunohistochemical methods, to visualize the penetration of secreted proteins in the tumor stroma”, says Daniel Öhlund.

The images show the nucleus and HER2 respectively, in exactly the same cells and the same tissue section.

 

 

Want to learn more? Please contact Product Specialist Tim Hofvendahl

We have several ongoing studies, and are interested in approaching more research teams for collaboration to identify where our product can have the greatest impact.

If you are interested in discussing a prospective collaboration and would like to know more about our technology, please contact our Product Specialist Tim Hofvendahl, e-mail: th@lumito.se, ph: +46 72 572 10 90 for further information.